Merged co-localization images are shown to the right.
( d) Staining images of cells fixed immediately after stretch release showing the membrane (pEYFP-mem transfection), paxillin and actin. ( c) Confocal vertical slice from a pEYFP-mem-transfected cell before (top) and after (bottom) application of 6% stretch for 3 min. ( b) Quantification of reservoir fluorescence after stretch release (1: initial fluorescence, 0: background). ( a) pEYFP-mem-transfected cells before, during and after constant stretch application during 3 min. ( f) % of cells showing membrane lysis after 3 min of application of medium with different osmolarity ( n=50 cells). ( e) % of cells showing membrane tearing after 3 min of constant stretch application ( n=70 cells). ( d) Corresponding quantification of the increase in cell volume and required membrane area ( n= 5 cells). ( c) Confocal slice showing a cell before (green) and after (red) application of medium with 50% osmolarity for 3 min. Yellow arrows indicate membrane ruffles, which either remain or flatten after applying 50 or 0% hypo-osmotic medium, respectively. Cells submitted to 0% osmolarity (de-ionized water) for 3 min sometimes rounded and flattened membrane ruffles (middle panel) and sometimes underwent membrane lysis (right panel). ( b) Cells transfected with pEYFP-mem before and after reducing medium osmolarity to either 50 or 0% of original medium. Yellow arrow indicates a membrane ruffle flattened by stretch. We trust this answers your question satisfactorily.( a) Cells transfected with pEYFP-mem before (top panel) and after (middle and bottom panels) applying different magnitudes of constant stretch. The key items are the semicolons at the end of each subpart and the word "and" at the end of (2) which tie the three parts into one requirement. Provides adequately for prevention of damage to the pipe by external forces.
Requires that mains be installed in a common trench with other utility lines.Establishes a minimum cover of less than 24 inches.
#ROBERTO ARTOLLA ASTELLIA INSTALL#
Thank you for your inquiry of October 2, 1979, (Reference NOTA GRIP/NT No.2373( regarding our pipeline safety regulation section 192.327(d). Thanking you in advance for your all cooperation and hoping a prompt answer, we remain Out also with the requirement of paragraph (2) or with paragraph (3), or with both Establishes a minimum cover of less than 24 inches " or if the installation must carry.Only the first requirement, that is: "(d) A main may be installed with less than 24 inches of cover if We ask you to inform us if a main might be installed with less then 24 inches of cover with Our duties include the analysis and studies of standards and specifications toīe complied with within the scope of our Company. Has del Estado is a State Owned Utility in charge of the production, transport,Ĭommercialization and distribution of gaseous hydrocarbons (Natural Gas and LPG) all over theĪrgentine country. Interpretation Response Details Response Publish Date: 11-07-1979 Below is the interpretation response detail and a list of regulations sections applicable to this response.
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